Determination of vomitoxin in corn flour by multifunctional purification column and liquid chromatography

Application of vomitoxin in corn flour

Sample extraction：

Weigh 5g corn flour sample (crushed and screened for 1mm) into 50mL centrifuge tube, add 20mL extraction solution (84% acetonitrile-aqueous solution) and mix well

Ultrasonic extraction 30min, put into centrifuge (8000r/min centrifuge 20min), take supernatant as sample extraction solution

Sample purification：

 Step 1: Add 5mL of the sample extract to the test tube

 Step 2: Insert the rubber tip of the column into the test tube from the top and press the column down to the bottom of the test tube

 Step 3: Pour the purified sample extract from the upper part of the column into the sample bottle or EP tube

 Step 4: Take 2mL of purified extract, blow nitrogen to dry, redissolve with 1mL of initial mobile phase, filter through microporous membrane, and machine analysis

HPLCDetection condition：

 Mobile phase: methanol-water (20:80, volume ratio)

Chromatographic column: C18 (column length 250mm, column inner diameter 4.6mm, packing particle size 5μm)

Flow rate: 1.0mL/min

 column temperature: 35℃

 Injection size: 50μL

 Ultraviolet detection: wavelength 218nm

Experimental result：

Vomitoxin liquid chromatography

(DON concentration is 1000ng/mL)

Liquid chromatogram of quality control substance sample of corn flour

(DON concentration is 900ng/g)

The minimum detection limit, recovery rate and repeatability of corn flour samples

(DON concentration is 900ng/g)