Qingdao Zhenzheng Analytical
Instrument Co., LTD
Mobile: 15963292045
Email: qdzhenzheng@163.com
QQ: 2776447184
Add: Building 27, International Industrial Park,
Shandong University, No.669 East China Road,
High-tech Zone, Qingdao
Website: www.qdzhenzheng.com
Application of zearalenone in corn flour
Sample extraction:
Weigh 6g corn flour sample (crushed and screened 1mm) in a 50mL centrifuge tube, add 0.6g sodium chloride and 15mL extraction solution (90% acetonitrile - aqueous solution) and mix well
Ultrasonic extraction 30min, put into centrifuge (8000r/min centrifuge 20min), take supernatant as sample extraction solution
Sample purification:
Step 1: Add 5mL of the sample extract to the test tube
Step 2: Insert the rubber tip of the column into the test tube from the top and press the column down to the bottom of the test tube
Step 3: Pour the purified sample extract from the upper part of the column into the sample bottle or EP tube
Step 4: Take 2mL of purified extract, blow nitrogen to dry, redissolve with 0.5mL of initial mobile phase, filter through microporous membrane, machine analysis
HPLCDetection condition:
Mobile phase: acetonitrile-methanol-water (46:8:46, volume ratio)
Chromatographic column: C18 (column length 250mm, column inner diameter 4.6mm, packing particle size 5μm)
Flow rate: 1.0mL/min
column temperature: 35℃
Injection size: 50μL
Fluorescence detection: excitation wavelength: 274nm; Emission wavelength: 440nm
Experimental result:
Zearalenone liquid chromatography
(ZON concentration is 100ng/mL)
Liquid chromatogram of quality control substance sample of corn flour
(ZON concentration is 218.3ng/g)
The minimum detection limit, recovery rate and repeatability of corn flour samples
(ZON concentration is 218.3ng/g)
玉米赤霉烯酮 | 最低检出限(ng/g) | 回收率(%) | 重复性RSD(n=3,%) |
ZON | 5.0 | 86.7 | 2.49 |
Qingdao Zhenzheng Analytical Instrument Co., LTD
Tel: 15963292045 Email: qdzhenzheng@163.com
QQ: 2776447184 Add: Building 27, Shandong University International Industrial Park, No.669 East China Road, High-tech Zone, Qingdao
Technical support:浩瀚网络 Record number:鲁ICP备14019093号-1
Web address: www.qdzhenzheng.com