黄曲霉毒素检测

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Qingdao Zhenzheng Analytical

 Instrument Co., LTD

Mobile: 15963292045

Email: qdzhenzheng@163.com

QQ: 2776447184

Add: Building 27, International Industrial Park, 

Shandong University, No.669 East China Road, 

High-tech Zone, Qingdao

Website: www.qdzhenzheng.com


Determination of zearalenone in corn flour by multifunctional purification column and liquid chromatography

Determination of zearalenone in corn flour by multifunctional purification column and liquid chromatography


  • Determination of zearalenone in corn flour by multifunctional purification column and liquid chromatography

Application of zearalenone in corn flour

 

Sample extraction:

Weigh 6g corn flour sample (crushed and screened 1mm) in a 50mL centrifuge tube, add 0.6g sodium chloride and 15mL extraction solution (90% acetonitrile - aqueous solution) and mix well


Ultrasonic extraction 30min, put into centrifuge (8000r/min centrifuge 20min), take supernatant as sample extraction solution


Sample purification:

Step 1: Add 5mL of the sample extract to the test tube

Step 2: Insert the rubber tip of the column into the test tube from the top and press the column down to the bottom of the test tube

Step 3: Pour the purified sample extract from the upper part of the column into the sample bottle or EP tube

Step 4: Take 2mL of purified extract, blow nitrogen to dry, redissolve with 0.5mL of initial mobile phase, filter through microporous membrane, machine analysis


1551768507968816.png

HPLCDetection condition:

Mobile phase: acetonitrile-methanol-water (46:8:46, volume ratio)


Chromatographic column: C18 (column length 250mm, column inner diameter 4.6mm, packing particle size 5μm)


Flow rate: 1.0mL/min


column temperature: 35℃


Injection size: 50μL


Fluorescence detection: excitation wavelength: 274nm; Emission wavelength: 440nm


Experimental result:

2.png

Zearalenone liquid chromatography

(ZON concentration is 100ng/mL)


3.png

Liquid chromatogram of quality control substance sample of corn flour

(ZON concentration is 218.3ng/g)


The minimum detection limit, recovery rate and repeatability of corn flour samples

(ZON concentration is 218.3ng/g)


玉米赤霉烯酮

最低检出限(ng/g

回收率(%

重复性RSDn=3,%

ZON

5.0

86.7

2.49


Key word:MycotoxinPurificationColumns

1551752942177375.png

Qingdao Zhenzheng Analytical Instrument Co., LTD

Tel: 15963292045 Email: qdzhenzheng@163.com

QQ: 2776447184 Add: Building 27, Shandong University International Industrial Park, No.669 East China Road, High-tech Zone, Qingdao

Technical support:浩瀚网络     Record number:鲁ICP备14019093号-1  

Web address: www.qdzhenzheng.com           

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黄曲霉毒素检测

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