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SPME-GC/MS analysis of liquorice odor, a volatile component of traditional Chinese medicine

SPME-GC/MS analysis of liquorice odor, a volatile component of traditional Chinese medicine


  • SPME-GC/MS analysis of liquorice odor, a volatile component of traditional Chinese medicine


SPMEProbe item numberZZ-SPME-06-s

 

Analysis condition:

 

图片1.png

Sample: 2 g licorice sample in 20 ml headspace bottle


Extraction temperature: 50 ºC; Extraction time: 60 min Image 1.png


Desorption time: 2 min; Desorption temperature: 250 ºC


Sample inlet: GC


Injection method: manual


Inlet: 250 ºC shunt, shunt ratio: 20:1


Column: DB-5ms: 50 m × 250 μm × 0.25 μm


Programmed heating: Initial temperature 40 ºC, hold for 3 min, 10 ºC/min rise to 280 ºC, hold for 10 min


Acquisition mode: Full scan, scanning range: 40-500 m/z


Ion source: 230 ºC, MS quadrupole: 150 ºC


 图片4.png

 

 

Total ion flow chromatogram of licorice odor detected by SPME-GC/MS

Detection result:

    A total of 58 organic compounds were detected, including 12 alcohols, 11 aldehydes, 16 alkanes, 1 olefin, 1 aromatic hydrocarbon, 3 acids, 8 esters, 2 halogenated hydrocarbons, 2 ketones, 1 oxime and 1 amine, with their respective contents as shown in the following table. The test results showed that compound No. 11 had the highest content of caproic acid, accounting for 38.129% of the total.

SPME-GC/MS detection result

序号

化合物名称

保留时间

峰面积百分比总量(%

1

4-methyl-Cyclohexanol(4-甲基环己醇)

5.381

0.409

2

2-propenylidene-Cyclobutene(2-丙烯基环丁烷)

6.749

0.140

3

1,2,3-Butanetriol(1,2,3-丁三醇)

7.201

0.143

4

2,3-Butanediol(2,3-丁二醇)

7.371

0.096

5

Hexanal(己醛)

7.439

16.873

6

2-Hexenal(2-己烯醛)

8.591

0.740

7

1-Hexanol(1-己醇)

8.870

5.859

8

4-(1-methylethyl)-Cyclohexanol(4-1-甲基乙基) - 环己醇)

9.296

0.320

9

methoxy-phenyl-Oxime(甲氧基 - 苯基 - )

9.346

0.359

10

Heptanal(庚醛)

9.539

0.646

11

Hexanoic acid(己酸)

11.211

38.129

12

Octanal(辛醇)

11.467

0.358

13

Benzyl chloride(氯化苄)

11.755

4.052

14

 1-methyl-2-(1-methylethyl)-Benzene(1-甲基-2-1-甲基乙基) -  )

11.901

0.401

15

 4-methyl-3-Cyclohexene-1-carboxaldehyde(4-甲基-3-环己烯-1-甲醛)

12.051

0.202

16

3,5-Octadien-2-ol(3,5-辛二烯-2-)

12.104

0.581

17

3-O-Benzyl-d-glucose(3-O-苄基-D-葡萄糖)

12.222

0.182

18

2-Octenal(2-辛烯醛)

12.467

1.491

19

Terpineol-π(松油醇-π)

12.735

0.285

20

n-Butyric acid 2-ethylhexyl ester(正丁酸2-乙基己酯)

13.067

0.731

21

Nonanal(壬醛)

13.211

2.074

22

1-methyl-4-(1-methylethyl)-2-Cyclohexen-1-ol(1-甲基-4-1-甲基乙基)-2-环己烯-1-)

13.275

0.187

23

 π(1-ethenylpentyl)-πmethyl-Benzenemethanol(π(1-甲基戊基)-πmethyl-苯甲醇)

13.351

0.162

24

 1-methyl-3-Cyclohexene-1-carboxaldehyde(1-甲基-3-环己烯-1-甲醛)

13.612

0.334

25

2-Nonenal(2-壬烯醛)

14.139

0.905

26

4-methyl-1-(1-methylethyl)-Bicyclo[3.1.0]hex-3-en-2-one(4-甲基-1-1-甲基乙基)二环[3.1.0]-3--2-)

14.395

0.343

27

4-methyl-1-(1-methylethyl)-3-Cyclohexen-1-ol(4-甲基-1-1-甲基乙基)-3-环己烯-1-)

14.572

0.226

28

Tetradecane(十四烷)

14.664

0.262

29

Decanal(癸醛)

14.816

0.906

30

Hexanoic acid  3-tetradecyl ester(己酸3-十四烷基酯)

15.764

0.093

31

Nonadecane(十九烷)

16.135

0.130

32

Bicyclo[4.4.1]undeca-1,3,5,7,9-pentaene(二环[4.4.1]十一1,3,5,7,9五烯)

16.421

0.183

33

3-Cyclopentylpropionic acid, 6-ethyl-3-octyl ester(3-环戊丙酸,6-乙基-3-辛酯)

16.882

0.648

34

2,6,10-trimethyl-Tetradecane (2,6,10-三甲基十四烷)

17.117

0.066

35

2-Butyl-2,7-octadien-1-ol(2-丁基-2,7-辛二烯-1-)

17.206

0.531

36

Hexanoic acid  hexyl ester(己酸己酯)

17.327

0.265

37

4-Trifluoroacetoxypentadecane(4-三氟乙酰十五烷)

17.414

0.100

38

Tetradecane(十四烷)

17.512

0.683

39

Dodecanal(十二烷)

17.702

0.152

40

1-Octadecanesulphonyl chloride(1-十八烯磺酰氯)

18.514

0.176

41

Pentadecane(十五烷)

18.808

0.489

42

N,N-dimethyl-1-Dodecanamine(NN-二甲基-1- 十二烷基伯胺)

18.890

8.835

43

 2,6,10-trimethyl-Tetradecane(2,6,10-三甲基十四烷)

20.030

0.228

44

Tetradecanal(十四烷)

20.245

0.342

45

 8-propoxy-Cedrane(8-丙氧基-柏木烷)

20.556

0.181

46

2,2,6,7-Tetramethyl-10-oxatricyclo[4.3.0.1(1,7)]decan-5-one(2,2,6,7四甲基-10-氧杂双环[4.3.0.11,7]-5-)

21.052

0.154

47

Heptadecane(十七烷)

21.187

0.337

48

N,N-dimethyl-1-Tetradecanamine(NN-二甲基-1-十四烷)

21.284

1.787

49

Octadecanal(十八烷)

21.415

1.485

50

1,2-Benzenedicarboxylic acid, butyl octyl ester(1,2-苯二甲酸丁酯辛酯)

23.030

0.086

51

9,17-Octadecadienal (9,17-烯醛)

23.303

0.391

52

2'-hexyl-[1,1'-Bicyclopropyl]-2-octanoic acid,  methyl ester(2'-己基 - [1,1'-丙基] -2-辛酸甲酯)

23.364

0.063

53

n-Hexadecanoic acid(正十六烷酸)

23.956

2.080

54

Hexadecanoic acid  ethyl ester(十六烷酸乙酯)

24.257

0.113

55

Heptacosane(二十七烷)

24.324

0.220

56

Ethyl iso-allocholate(异胆酸乙酯)

25.058

0.249

57

N-Methyl-N-benzyltetradecanamine(N-甲基-N-苄基十四烷)

25.533

1.692

58

7-ethenyl-1,2,3,4,4a,4b,5,6,7,1-Phenanthrenecarboxylic acid, (7-乙基桥-12344a4b5671 - 菲羧酸)

27.568

0.847


Key word:Solidphasemicroextraction

1551752942177375.png

Qingdao Zhenzheng Analytical Instrument Co., LTD

Tel: 15963292045 Email: qdzhenzheng@163.com

QQ: 2776447184 Add: Building 27, Shandong University International Industrial Park, No.669 East China Road, High-tech Zone, Qingdao

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